The Columbia root knot nematode (CRKN), Meloidogyne chitwoodi Golden et al., is a major pest in the United States Pacific Northwest potato production areas. Both roots and tubers are attacked, leading to decreases in yield and quality. Resistance to CRKN was identified from wild diploid species Solanum bulbocastanum accession SB22 and was successfully introgressed into tetraploid potato breeding material. To improve breeding efficiency and to develop CRKN-resistant potato cultivars, we developed genomic resources for SB22. We have assembled whole genome sequence of SB22 using Illumina and PacBio sequencing technologies and bioinformatics tools. Further to understand the diversity associated with different pathotypes of the nematode, draft genome of M. Chitwoodi Race 1, Race 2 and Race 1 pathotype Roza was developed using PacBio Sequencing. The genomic information developed will be used for marker development and breeding resistant varieties.