CANCELLED: Examination of stylar and pollen-based components of self-compability
Date & Time
Monday, July 18, 2022, 2:30 PM

Potato breeding is being redirected to conduct breeding at a diploid level to take advantage of the innate efficiencies of a simpler genetic state. For diploid breeding to succeed we need a robust and predictable self-compatibility (SC) system to exploit the development of inbred lines. Sli-based SC is pollen-based and has been introgressed from Solanum chacoense into the Michigan State University breeding program. Through gene editing, S-RNase and HT-B, stylar-based SC genes, have been knocked out in a diploid breeding line. A cross was made between two self-compatible breeding lines (DRH195-158 x MSEE737-05) so that the F1 progeny could be phenotyped for SC and genotyped for the three SC genes. Based on PCR genotyping for Sli, the gene segregated in a 1:1 fashion, indicative of the zygosity of the parents. Of the 40 plants self-pollinated in this study, 30 of them gave fruit, characteristic of their SC status, and 98% of fruit gave seed. The allelic state of the two edited genes, S-RNase and HT-B will be characterized through sequencing, and based on these results as well as the previous results, an F2 population will be created to conduct QTL analysis for SC. Additionally, another gene editing study is being conducted to knock out the HT-A gene, the complement of HT-B, to create double HT-A/HT-B knockout lines and eventually triple HT-A/HT-B/S-RNase knockout lines. We expect this study to contribute to our understanding of SC in diploid potato and lead to the identification of self-compatible germplasm that can be used in diploid breeding programs.

Session Type
Parent Session
7/18 - Concurrent Sessions A