Genome sequencing of adapted non-inbred diploid potato clones
Date & Time
Tuesday, July 19, 2022, 9:00 AM
Helen Tai

Cultivated potato is a vegetatively-propagated crop, and current commercial varieties are autotetraploid with high levels of heterozygosity and genetic complexity. Reducing the ploidy and breeding potato at the diploid level can increase efficiency for genetic improvement including greater ease of introgression of diploid wild relatives and more efficient use of genomics and markers in selection. More recently, development of F1 hybrid breeding opens another facet of diploid potato breeding with the potential for hybrid vigor and propagation using true potato seed. Diploid F1 hybrid breeding requires self-compatible diploid clones for development inbred lines. Severe inbreeding depression was documented for diploid potato and presents challenges for F1 hybrid breeding. The current study provides genome sequence resources for nine legacy non-inbred adapted diploid potato clones developed at Agriculture and Agri-Food Canada by retired breeder Hielke (Henry) De Jong. De novo genome sequence assembly using 10x Genomics and Illumina sequencing technologies show the genome sizes ranged from 714 Mbp to 952 Mbp. The genomes were analyzed for dysfunctional genes that can contribute to inbreeding depression. Genotyping of loci controlling early tuberization (StCDF1) and self-incompatibility (Sli) was also done. These resources will be useful in advancing genome design for diploid potato breeding.

Session Type
Parent Session
7/19 - Concurrent Sessions D