AJPR 98:304-314 cont.: A higher high-throughput method for generating dihaploids from tetraploid potato
Date & Time
Wednesday, July 20, 2022, 10:00 AM
Cari Schmitz Carley

Global interest in reinventing potato as a diploid, hybrid crop has been buoyed in the past several years by major enabling research advancements. Aardevo North America targets the development of diploid, hybrid cultivars for the French fry processing market. A bottleneck in these efforts has been repackaging traits from tetaploid potato cultivars into dihaploid plants via prickle pollination inductions. Induction frequencies are generally low, and fertility problems in dihaploids can further limit the movement of genes from tetraploid cultivars into diploid breeding programs. A high throughput method for generating dihaploids was recently published in the American Journal of Potato Research. Here, we report additional modifications to the cut stem (“decapitationâ€) method . The modifications improved throughput, and moderate to high induction success rates were observed within the North American russeted processing germplasm. Across over 20 tetraploid russets , induction success rates ranged from 4 to 84%, with a mean of 32%, expressed as a proportion of true diploids in unspotted seeds. Altogether, 10% of cut stems resulted in a dihaploid. While it has been suggested that male fertility in the tetraploid can be indicative of inducibility, this trend was not observed. Given the appropriate timing and infrastructure, prickle pollination on field-grown cut stems is a highly scalable and efficient method of ploidy reduction relative to working with potted plants in the greenhouse.

Session Type
Parent Session
7/20 - Concurrent Sessions H