Solanum microdontum (mcd) obtained from the USDA Potato genebank in Sturgeon Bay, WI was used in hybridizations to cultivated potato (tbr) due to previous screening assays that showed low tuber greening, an important tuber quality trait. A tetraploid mcd-tbr hybrid, A13396-31 was generated and characterized as having resistance to both tuber greening and the accumulation of tuber glycoalkaloids following extended illumination. This unique interspecific hybrid was hybridized with the potato variety Castle Russet to generate a family A201085 composed of approximately 270 individuals. This mapping population was genotyped with the Illumina Infinium SolCAP potato SNP V3 array for genetic analysis. Potato genetic linkage maps were constructed by MAPpoly, which is an R-package, focusing on the creation of linkage maps for polyploids. QTL analyses was employed using the phenotypic data collected on individuals from A201085 in the greenhouse which included leaf edema, plant growth characteristics, and purple coloration in the stem and leaves. Significant QTLs associated with these vine morphological characteristics were observed on chromosomes 3 and 4. Additionally, a subpopulation composed of 141 clones, which produced sufficient tuber numbers for tuber greening evaluation, was also assessed for significant QTLS with preliminary findings to be reported. Experimentation will be repeated in 2022 to collect a second year of data and to confirm and further refine the QTL results.