There is a lack of quantitative identification and evaluation of the nutritional needs of the potato production systems. Therefore, a greater comprehensive understanding of nutrient uptake and partitioning is necessary to facilitate nutrient management and nitrogen use efficiency. Hence, the objective of the study was to evaluate major macro and micronutrient accumulation and partitioning in current modern Russet varieties grown in Wisconsin, USA. In the year 2020, Russet Burbank, Goldrush, Russet Norkotah, and Silverton planted under nitrogen unlimited and stressed conditions were sampled ten times throughout the growing season and divided into three plant tissue fractions for quantification of nutrient uptake and partitioning. Accumulation (per ha) of nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, zinc, iron, copper, and manganese required to produce maximum yield under nitrogen fertilized as well as unfertilized conditions will be identified. Similarly, the in-season biomass and nutrient uptake patterns of these varieties along with their partitioning dynamics in the above-ground stem and leaves and below-ground tubers will also be evaluated. This study will provide a comprehensive understanding of the fundamental differences in the nutrient and dry matter biomass uptake and partitioning dynamics of the potato varieties under nitrogen stressed and non-stressed conditions. Hence, providing evidence if the most common Russet varieties can be managed similarly or not.