Early vine kill in seed potato production is a useful strategy to reduce tuber size, but it has also been reported to reduce incidence of viruses such as Potato virus Y (PVY) in daughter tubers. Whether this strategy is effective in Idaho has not been previously demonstrated. In 2017, 2020 and 2021, we examined the impact of two calendar-based vine kill timings (early, when natural senescence was 5 to 10%; and conventional, three weeks later) on PVY incidence in daughter tubers of two potato cultivars, â€˜Russet Burbankâ€™ and â€˜Ranger Russet.â€™ The vine-kill timing field trial was established each year at the UI Aberdeen Research & Extension Center in southeastern Idaho. Treatments were arranged in a randomized complete block design with six (2017) or four (2020, 2021) replications. Plots were comprised of two 20-ft rows with 36-in between row spacings. Certified seed potatoes with 0% PVY were sown at 12-in within row spacing (40 plants per plot); and plots were surrounded by Russet Burbank border rows. At harvest (three weeks after conventional vine kill timing), 40 daughter tubers (one per plant) were collected from each plot and subjected to a â€œgrow outâ€ either in Hawaii (2017, 2021) or at the University of Idaho Kimberly Research & Extension Center in southcentral Idaho (2020). During the grow out, one leaf from each emerged plant was picked and tested individually or in bulk (up to five leaves per test well) via DAS-ELISA to estimate PVY incidence. Data were analyzed using the general linear mixed model procedure in SAS 9.4, and estimated means were separated using pairwise comparisons (alpha=0.05). Differences in post-harvest PVY were not apparent for vine kill time (P = 0.1156), but Ranger Russet had higher PVY incidence (P < 0.0001). Impact on yield and profile size will be discussed.