Potato breeders are testing the efficacy of inducing a reduction in ploidy in cultivated potato to breed at the diploid level. Breeding potato as a diploid crop comes with many benefits, including simplifying the breeding pipeline and making it easier to apply genetic tools. However, it is unclear how similar induced diploids will be to their tetraploid counterparts. Here, we present a comparison of diploid and tetraploid leaf spectra. Spectroscopy offers a cheap and easy method for comparing the leaf chemistry, which has a large impact on a plant’s overall health and fitness. Leaf chemistry can point to underlying traits of importance such as nitrogen uptake efficiency. We used a spectroradiometer from the Spectral Vista Corporation to acquire spectra from the 400nm-2500nm range, which includes visible and small infrared wavelengths. We grew diploid and tetraploid plants from tissue culture in the greenhouse in a randomized complete block design. We took spectral measurements from leaf tissue 5 weeks after planting. We show that diploid and tetraploid spectra have distinct, but overlapping, distributions. We also examined the wavelengths that showed the most divergence between ploidies to explore the underlying chemistry which differed between the two ploidies.