Effect of Brassica juncea seed meal extract combined with 4-hydroxybenzyl alcohol on control of Globodera pallida.

Wednesday, July 28, 2021
12:00 PM - 12:15 PM


The pale cyst nematode (PCN), Globodera pallida, is a highly specialized, economically important pest of potato. The control of G. pallida is difficult because of its ability to remain dormant in the soil for about 20 years in the absence of its host. The specificity of G. pallida hatching makes this life stage a potential target for designing efficient integrated control strategies. We have demonstrated that 4-hydroxybenzyl alcohol (HBA) enhances G. pallida egg hatch by 60% compared to the non-treated control. The increase in the hatch in the presence of potato root diffusate is speculated to be due to an increase in egg permeability. Contrary to HBA, Brassica juncea seed meal extract, through the production of volatile allyl isothiocyanate, inhibits G. pallida egg hatch and reproduction. This study aims to determine the efficacy of B. juncea seed meal extract to control G. pallida when combined with HBA. The impact of 0, and 4.48 ton/ha HBA and four rates (0, 0.14, 0.56, 1.12 ton/ ha) of B. juncea seed meal extract on egg hatch and viability were determined under laboratory, and greenhouse conditions. The data showed reduced nematode hatch and viability with all the tested rates of B. juncea seed meal extract. The combination of HBA and B. juncea seed meal extract further reduced percentage hatch and viability than seed meal extract alone. Lower percentage of egg hatch and viability with the combination indicates that HBA was able to increase the vulnerability of G. pallida eggs to B. juncea seed meal extract thus, killing more nematodes. Moreover, further experiment to assess the impact of the combination on G. pallida reproduction, determined by bioassay with susceptible potato cultivar, is underway.

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