We have developed a population of diploid recombinant inbred lines for use in mapping in potato. The doubled monoploid DM 1-3 was crossed as a female to the inbred S. chacoense clone M6. A single F1 plant was self-pollinated to generate a large F2 population. F2 plants were self-pollinated to the F5 or F6 generation. The only selection criterion during the development of the inbreds was the ability to produce seeds following self-pollination. Attrition was high in each generation; in most cases, lines were lost due to flower and fertility abnormalities. We have generated nearly 100 inbred lines, each of which carries a mosaic of the DM 1-3 and M6 genomes. This population is highly variable for vine, tuber, and fertility traits, so we expect it to be a useful mapping resource. It will be available clonally through the US Potato Genebank.